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Ube2t 抗体

This 兔 多克隆 antibody specifically detects Ube2t in WB, ELISA, IP 和 IF. It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN5700967
发货至: 中国
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Quick Overview for Ube2t 抗体 (ABIN5700967)

抗原

See all Ube2t 抗体
Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

适用

  • 42
  • 14
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 37
  • 4
  • 1

克隆类型

  • 36
  • 6
多克隆

标记

  • 26
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Ube2t antibody is un-conjugated

应用范围

  • 30
  • 23
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)
  • 原理

    UBE2T antibody

    免疫原

    ubiquitin-conjugating enzyme E2T (putative)

    亚型

    IgG
  • 应用备注

    WB : 1:200-1:2000,IP:1:200-1:2000,IF:1:50-1:500

    说明

    Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09185(UBE2T antibody) at dilution of 1:800

    限制

    仅限研究用
  • 缓冲液

    PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze / thaw cycles.

    储存条件

    -20 °C

    储存方法

    -20°C for 12 months

    有效期

    12 months
  • 抗原

    Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

    别名

    UBE2T

    背景

    Synonyms: Ubiquitin-conjugating enzyme E2 T|Cell proliferation-inducing gene 50 protein|E2 ubiquitin-conjugating enzyme T|Ubiquitin carrier protein T|Ubiquitin-protein ligase T|UBE2T

    Background: The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

    分子量

    24kDa

    UniProt

    Q9NPD8

    途径

    Chromatin Binding
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