Ube2t 抗体
Quick Overview for Ube2t 抗体 (ABIN5700967)
抗原
See all Ube2t 抗体适用
宿主
克隆类型
标记
应用范围
-
-
免疫原
- ubiquitin-conjugating enzyme E2T (putative)
-
亚型
- IgG
-
-
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) antibody
Ube2t 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1E12-4A3 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) antibodyUbe2t 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2A12-4F11 unconjugated
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (AA 1-197) antibodyUbe2t 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (C-Term) antibodyUbe2t 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (C-Term) antibodyVerified Ube2t 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (Internal Region) antibodyUbe2t 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (Internal Region) antibodyUbe2t 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2T (Ube2t) (N-Term) antibodyUbe2t 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- WB : 1:200-1:2000,IP:1:200-1:2000,IF:1:50-1:500
-
说明
-
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09185(UBE2T antibody) at dilution of 1:800
-
限制
- 仅限研究用
-
-
-
缓冲液
- PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freeze / thaw cycles.
-
储存条件
- -20 °C
-
有效期
- 12 months
-
-
- Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))
-
别名
- UBE2T/HSPC150
-
背景
- Synonyms:HSPC150, PIG50, UBE2T, Ubiquitin carrier protein T, Ubiquitin protein ligase T Background:The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
-
分子量
- 24kDa
-
UniProt
- Q9NPD8
-
途径
- Chromatin Binding
抗原
-
