SCRIB 抗体 (AA 172-409)
Quick Overview for SCRIB 抗体 (AA 172-409) (ABIN5693286)
抗原
See all SCRIB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 172-409
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原理
- Anti-SCRIBBLE Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-SCRIBBLE Antibody Picoband® (ABIN5693286). Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).
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亚型
- IgG
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anti-Scribbled Homolog (SCRIB) antibody
SCRIB 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Scribbled Homolog (SCRIB) (AA 382-410), (N-Term) antibodySCRIB 适用: 人 WB 宿主: 兔 Polyclonal RB39490 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
1. L E Dow, J S Kauffman, J Caddy, A S Peterson, S M Jane, S M Russell & P O Humbert (April 2007). "The tumour-suppressor Scribble dictates cell polarity during ed epithelial migration: regulation of Rho GTPase recruitment to the leading edge". Oncogene. 26 (16): 2272-82. 2. Yi Qin, Christopher Capaldo, Barry M. Gumbiner & Ian G. Macara (December 2005). "The mammalian SCRIB polarity protein regulates epithelial cell adhesion and migration through E-cadherin". JCB. 171 (6): 1061-71. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- SCRIB (Scribbled Homolog (SCRIB))
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别名
- SCRIB
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背景
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Synonyms: Protein scribble homolog, Scribble, hScrib, Protein LAP4, SCRIB, CRIB1, KIAA0147, LAP4, SCRB1, VARTUL
Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
Background: SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and epithelial polarization. Mechanistically, the human homolog is a scaffold protein linked to cellular differentiation centered on the regulation of epithelial as well as neuronal morphogenesis. Deficiency in SCRIB impairs many aspects of cell polarity and cell movement. SCRIB is also likely involved in establishing apical-basal polarity as well as progression from the G1 phase to S phase in the cell cycle as a result of its relationship with cell proliferation and exocytosis.
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分子量
- 240 kDa
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基因ID
- 23513
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UniProt
- Q14160
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途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Tube Formation, Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
抗原
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