ADO 抗体 (AA 49-261) (DyLight 488)
Quick Overview for ADO 抗体 (AA 49-261) (DyLight 488) (ABIN7354745)
抗原
See all ADO 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 49-261
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原理
- Anti-Human ADO DyLight® 488 conjugated Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Human ADO DyLight® 488 conjugated Antibody -Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
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免疫原
- E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.
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亚型
- IgG
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anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) antibody
ADO 适用: 人, 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) antibodyADO 适用: 人, 小鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) (AA 137-270) antibodyADO 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) (AA 206-233), (C-Term) antibodyADO 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB30095 unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) (AA 206-233), (C-Term) antibodyADO 适用: 人, 小鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow Cytometry (Fixed), 1-3 μg/1x106 cells1. Dominy, J. E., Jr., Simmons, C. R., Hirschberger, L. L., Hwang, J., Coloso, R. M., Stipanuk, M. H. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.J. Biol. Chem. 282: 25189-25198, 2007.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
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- ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))
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别名
- ADO
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背景
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Synonyms: 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase, ADO, C10orf22
Tissue Specificity: Expressed only in a subset of type 2 skeletal muscle fibers.
Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
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分子量
- 39 kDa
抗原
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