NSF 抗体 (AA 620-744)
Quick Overview for NSF 抗体 (AA 620-744) (ABIN5693126)
抗原
See all NSF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 620-744
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原理
- Anti-NSF Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-NSF Antibody Picoband® (ABIN5693126). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- E. coli-derived human NSF recombinant protein (Position: N620-D744).
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亚型
- IgG
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anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 590-744) antibody
NSF 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 1-250) antibodyNSF 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibodyNSF 适用: 大鼠, 小鼠 WB, ELISA, IP, IHC, ICC 宿主: 小鼠 Monoclonal 83-11 unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 668-696), (C-Term) antibodyNSF 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB41933 unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (Internal Region) antibodyNSF 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 733-744) antibodyNSF 适用: 人, 大鼠, 小鼠, Hamster WB, ELISA, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (C-Term) antibodyNSF 适用: 人, 大鼠, 小鼠, Cow, Pig, 兔, 犬, 马, 斑马鱼, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 1-744) antibodyNSF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 1-744) antibodyNSF 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 662-711) antibodyNSF 适用: 人, 大鼠, 小鼠, Cow, Hamster, Pig, 兔, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: NSF N-ethylmaleimide-sensitive factor". 2. Hoyle J, Phelan JP, Bermingham N, Fisher EM (Nov 1996). "Localization of human and mouse N-ethylmaleimide-sensitive factor (NSF) gene: a two-domain member of the AAA family that is involved in membrane fusion". Mammalian Genome. 7 (11): 850-2. 3. Wilson DW, Whiteheart SW, Wiedmann M, Brunner M, Rothman JE (May 1992). "A multisubunit particle implicated in membrane fusion". The Journal of Cell Biology. 117 (3): 531-8. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
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别名
- NSF
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背景
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Synonyms: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF
Background: N-ethylmaleimide-sensitive factor, also known as NSF, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.
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分子量
- 82 kDa
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基因ID
- 10874
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UniProt
- P46459
抗原
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