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NSF 抗体 (AA 620-744)

NSF 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5693126
发货至: 中国
  • 抗原 See all NSF 抗体
    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
    抗原表位
    • 14
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 620-744
    适用
    • 46
    • 30
    • 30
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠
    宿主
    • 60
    • 3
    • 1
    克隆类型
    • 63
    • 1
    多克隆
    标记
    • 32
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NSF antibody is un-conjugated
    应用范围
    • 52
    • 26
    • 12
    • 12
    • 9
    • 8
    • 8
    • 6
    • 5
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    原理
    Anti-NSF Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins.
    产品特性
    Anti-NSF Antibody Picoband® (ABIN5693126). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    免疫原
    E. coli-derived human NSF recombinant protein (Position: N620-D744).
    亚型
    IgG
    Top Product
    Discover our top product NSF Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    ELISA, 0.1-0.5 μg/mL
    1. "Entrez Gene: NSF N-ethylmaleimide-sensitive factor". 2. Hoyle J, Phelan JP, Bermingham N, Fisher EM (Nov 1996). "Localization of human and mouse N-ethylmaleimide-sensitive factor (NSF) gene: a two-domain member of the AAA family that is involved in membrane fusion". Mammalian Genome. 7 (11): 850-2. 3. Wilson DW, Whiteheart SW, Wiedmann M, Brunner M, Rothman JE (May 1992). "A multisubunit particle implicated in membrane fusion". The Journal of Cell Biology. 117 (3): 531-8.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
    别名
    NSF (NSF 产品)
    背景

    Synonyms: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF

    Background: N-ethylmaleimide-sensitive factor, also known as NSF, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.

    分子量
    82 kDa
    基因ID
    10874
    UniProt
    P46459
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