PEBP1 抗体
Quick Overview for PEBP1 抗体 (ABIN5693043)
抗原
See all PEBP1 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-PBP/PEBP1 Antibody Picoband®
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序列
- DHRGKFKVAS FRKKYELRAP VAGTCYQAEW DDYVPKLYEQ
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-PBP/PEBP1 Antibody Picoband® (ABIN5693043). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human PBP, which shares 87.5% and 80% amino acid (aa) sequence identity with mouse and rat PBP, respectively.
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亚型
- IgG
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anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 2-187) antibody
PEBP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 2-187) antibodyPEBP1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (C-Term) antibodyVerified PEBP1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (C-Term) antibodyPEBP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 1-187) antibodyPEBP1 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 97-128) antibodyPEBP1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) antibodyPEBP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 1-187) antibodyPEBP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D3-1B6 unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 1-30), (N-Term) antibodyPEBP1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3718 unconjugated
anti-Phosphatidylethanolamine Binding Protein 1 (PEBP1) (AA 137-167) antibodyPEBP1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3719 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells1. Chatterjee, D., Bai, Y., Wang, Z., Beach, S., Mott, S., Roy, R., Braastad, C., Sun, Y., Mukhopadhyay, A., Aggarwal, B. B., Darnowski, J., Pantazis, P., Wyche, J., Fu, Z., Kitagwa, Y., Keller, E. T., Sedivy, J. M., Yeung, K. C. RKIP sensitizes prostate and breast cancer cells to drug-induced apoptosis. J. Biol. Chem. 279: 17515-17523, 2004. 2. Hengst, U., Albrecht, H., Hess, D., Monard, D. The phosphatidylethanolamine-binding protein is the prototype of a novel family of serine protease inhibitors. J. Biol. Chem. 276: 535-540, 2001. 3. Yeung, K., Seitz, T., Li, S., Janosch, P., McFerran, B., Kaiser, C., Fee, F., Katsanakis, K. D., Rose, D. W., Mischak, H., Sedivy, J. M., Kolch, W. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature 401: 173-177, 1999. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))
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别名
- PEBP1
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背景
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Synonyms: Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP, Hippocampal cholinergic neurostimulating peptide, HCNP, PEBP1, PBP, PEBP
Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
Background: PEBP1 (Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein (PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al (2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.
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分子量
- 21 kDa
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基因ID
- 5037
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UniProt
- P30086
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途径
- Feeding Behaviour
抗原
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