Splicing Factor 1 抗体 (AA 160-266)
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Quick Overview for Splicing Factor 1 抗体 (AA 160-266) (ABIN5692946)
抗原
See all Splicing Factor 1 (SF1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 160-266
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原理
- Anti-splicing factor 1/SF1 Antibody
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-splicing factor 1/SF1 Antibody (ABIN5692946). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).
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亚型
- IgG
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anti-Splicing Factor 1 (SF1) (AA 1-110) antibody
SF1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-Splicing Factor 1 (SF1) (AA 1-111) antibodySF1 适用: 人 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-Splicing Factor 1 (SF1) (Ser82) antibodySF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 517-566) antibodySF1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 11-30), (N-Term) antibodySF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, IHC (fro), FACS 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (N-Term) antibodySF1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (Ser82) antibodySF1 适用: 人, 小鼠 WB, ELISA, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (pSer82) antibodySF1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (N-Term) antibodySF1 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Splicing Factor 1 (SF1) (AA 65-114) antibodySF1 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 马, 豚鼠, 斑马鱼, 猴, Pig, Bat, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
ELISA, 0.1-0.5 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Splicing Factor 1 (SF1)
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别名
- SF1
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背景
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Synonyms: Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162
Tissue Specificity: Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
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分子量
- 68-80 kDa
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基因ID
- 7536
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UniProt
- Q15637
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途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
抗原
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