EEA1 抗体
Quick Overview for EEA1 抗体 (ABIN5692856)
抗原
See all EEA1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- Anti-EEA1 Antibody Picoband®
-
序列
- RENQSLQIKH TQALNRKWAE DNEVQN
-
交叉反应 （详细）
- No cross-reactivity with other proteins.
-
产品特性
- Anti-EEA1 Antibody Picoband® (ABIN5692856). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human EEA1, identical to the related mouse and rat sequences.
-
亚型
- IgG
-
-
anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibody
EEA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibodyEEA1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1182-1411) antibodyEEA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 734-875) antibodyEEA1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (C-Term) antibodyEEA1 适用: 人, 小鼠, 大鼠, 犬, Hamster, 兔, 马, Cow, 猴, Pig, 豚鼠, 小鸡, Avian, Cat, 驴, 山羊, 绵羊 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1312-1411) antibodyEEA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) antibodyEEA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 1251-1350) antibodyEEA1 适用: 人, Hamster ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (N-Term) antibodyEEA1 适用: 人, 小鼠, 大鼠, 犬, 兔, 马, Cow, 豚鼠 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Early Endosome Antigen 1 (EEA1) (AA 2-13) antibodyEEA1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL
Immunohistochemistry, 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
1. Barysch SV, Aggarwal S, Jahn R, Rizzoli SO (Jun 2009). "Sorting in early endosomes reveals connections to docking- and fusion-associated factors". Proceedings of the National Academy of Sciences of the United States of America. 106 (24): 9697-702. 2. Mishra A, Eathiraj S, Corvera S, Lambright DG (Jun 2010). "Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1)". Proceedings of the National Academy of Sciences of the United States of America. 107 (24): 10866-71. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- EEA1 (Early Endosome Antigen 1 (EEA1))
-
别名
- EEA1
-
背景
-
Synonyms: Early endosome antigen 1, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2, EEA1, ZFYVE2
Tissue Specificity: Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Background: The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds ly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo.
-
分子量
- 170 kDa
-
基因ID
- 8411
-
UniProt
- Q15075
-
途径
- Toll-Like Receptors Cascades
抗原
-
