HLA Class I Heavy Chain 抗体
Cited in 2+ publications.
The 小鼠 单克隆 anti-HLA Class I Heavy Chain antibody (Clone HCA2) (ABIN5691101) specifically detects HLA Class I Heavy Chain in FACS, ELISA, IP, WB, IHC (fro), EM, ICC 和 IHC (p).
The antibody is reactive with 人 samples.
1 image
(2 references)
产品编号 ABIN5691101
发货至:
中国
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Quick Overview for HLA Class I Heavy Chain 抗体 (ABIN5691101)
抗原
HLA Class I Heavy Chain
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
HCA2
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特异性
- The mouse monoclonal antibody HCA2 recognize HLA class I heavy chains. The reactivity spectrum of HCA2 is composed of all HLA-A chains (except HLA-A24), as well as some HLA-B, HLA-C, HLA-E, HLA-F, and HLA-G chains.
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免疫原
- HCA2 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
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亚型
- IgG1
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anti-HLA Class I Heavy Chain antibody
适用: 人 FACS, ELISA, IP, WB, IHC (fro), EM, ICC, IHC (p) 宿主: 小鼠 Monoclonal HC10 unconjugated
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应用备注
- The antibody HCA2 reacts preferentially with HLA-A locus heavy chains. HCA2 was raised against free class I heavy chains of HLA, to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of formalin-fixed, paraffin-embedded sections, and cryo-immuno-electron microscopy. HCA2 indeed retains strong reactivity with free class I heavy chains in Western blots. HCA2 in particular reacts in a locus-specific manner by biochemical criteria. Conditions are described for use of HCA2 in immunohistochemical staining of formalin-fixed, paraffin-embedded sections (see references). HCA2 also produces strong reactivity in immuno-electron microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigens. Optimal antibody dilutions for the different applications should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
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限制
- 仅限研究用
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缓冲液
- Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C, or in small aliquots at -20°C.
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: "Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids. ..." in: Cell, Vol. 174, Issue 6, pp. 1586-1598.e12, (2019) (PubMed).
: "Increased PD-L1 and T-cell infiltration in the presence of HLA class I expression in metastatic high-grade osteosarcoma: a rationale for T-cell-based immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 66, Issue 1, pp. 119-128, (2017) (PubMed).
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: "Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids. ..." in: Cell, Vol. 174, Issue 6, pp. 1586-1598.e12, (2019) (PubMed).
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- HLA Class I Heavy Chain
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背景
- The HLA class I gene family is composed of a group of genes whose products encode cell surface glycoproteins of MW 40-45 kDa, associated non-covalently with the beta-2-microglobulin light chain. They include the three polymorphic molecules HLA-A, -B, and -C, which are ubiquitously expressed and which are able to present intracellular peptides to cytotoxic T cells. Three additional class I genes are known, commonly referred to as non-classical or class Ib genes, all highly homologous to the other class I genes and all of which associate with beta-2-microglobulin light chain. In humans, each of the class Ib genes appears to exhibit a distinct pattern of expression in developing and adult tissues. HLA-E transcripts are distributed widely in adult tissues and have also been found in the placenta and fetal liver. In the adult, the presence of HLA-F has been shown in skin, resting T cells, and B cells, whereas its expression during development has been reported in fetal liver and at low levels in placenta and extra-placental tissues. HLA-G was originally thought to be expressed only in certain populations of placental trophoblasts, but low levels have also been found in a variety of human tissues. Recently it was shown that HLA class I expression in breast cancer cells can have a predictive value for chemotherapy response.
抗原
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