HLA Class I Heavy Chain 抗体
There are 10+ publications for this product available. The 小鼠 单克隆 anti-HLA Class I Heavy Chain antibody is suitable to detect HLA Class I Heavy Chain in samples from 人. It has been validated for FACS, ELISA, IP, WB, IHC (fro), EM, ICC 和 IHC (p).
(10 references)
产品编号 ABIN5691100
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for HLA Class I Heavy Chain 抗体 (ABIN5691100)
抗原
HLA Class I Heavy Chain
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
克隆位点
HC10
-
-
特异性
- The mouse monoclonal antibody HC10 recognizes HLA class I heavy chains. HC10 reacts mostly with HLA-B and HLA-C heavy chains and some HLA-A (HLA-A10, HLA-A28, HLA-A29, HLA-A30, HLA-A31, HLA-A32, HLA-A33).
-
免疫原
- HC10 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
-
亚型
- IgG2a
-
-
anti-HLA Class I Heavy Chain antibody
适用: 人 FACS, ELISA, IP, WB, IHC (fro), EM, ICC, IHC (p) 宿主: 小鼠 Monoclonal HCA2 unconjugated
-
-
应用备注
- HC10 was raised against free class I heavy chains of HLA antigens to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of formalin-fixed, paraffin-embedded sections, and cryo-immuno-electron microscopy. HC10 indeed retains strong reactivity with free class I heavy chains in Western blots. HC10 also produces strong reactivity in immuno-electron microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigen and can be used to specifically block free heavy chains (FHC), See reference 6. Optimal antibody dilutions for the different applications should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
-
限制
- 仅限研究用
-
-
-
缓冲液
- Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C, or in small aliquots at -20°C.
-
-
-
: "Mutations in the IFNγ-JAK-STAT pathway causing resistance to immune checkpoint inhibitors in melanoma increase sensitivity to oncolytic virus treatment." in: Clinical cancer research : an official journal of the American Association for Cancer Research, (2021) (PubMed).
: "Analysis of the Tumor Reactivity of Tumor-Infiltrating Lymphocytes in a Metastatic Melanoma Lesion that Lost Major Histocompatibility Complex Class I Expression after Anti-PD-1 Therapy." in: The Journal of investigative dermatology, Vol. 139, Issue 7, pp. 1490-1496, (2020) (PubMed).
: "Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids. ..." in: Cell, Vol. 174, Issue 6, pp. 1586-1598.e12, (2019) (PubMed).
: "Significance of tumor-infiltrating lymphocytes before and after neoadjuvant therapy for rectal cancer." in: Cancer science, Vol. 109, Issue 4, pp. 966-979, (2018) (PubMed).
: "HLA Class I Antigen Expression in Conjunctival Melanoma Is Not Associated With PD-L1/PD-1 Status." in: Investigative ophthalmology & visual science, Vol. 59, Issue 2, pp. 1005-1015, (2018) (PubMed).
: "Increased PD-L1 and T-cell infiltration in the presence of HLA class I expression in metastatic high-grade osteosarcoma: a rationale for T-cell-based immunotherapy." in: Cancer immunology, immunotherapy : CII, Vol. 66, Issue 1, pp. 119-128, (2017) (PubMed).
: "Many de novo donor-specific antibodies recognize β2 -microglobulin-free, but not intact HLA heterodimers." in: HLA, Vol. 87, Issue 5, pp. 356-66, (2017) (PubMed).
: "Monitoring native HLA-I trimer specific antibodies in Luminex multiplex single antigen bead assay: Evaluation of beadsets from different manufacturers." in: Journal of immunological methods, Vol. 450, pp. 73-80, (2017) (PubMed).
: "Conformational Variants of the Individual HLA-I Antigens on Luminex Single Antigen Beads Used in Monitoring HLA Antibodies: Problems and Solutions." in: Transplantation, Vol. 101, Issue 4, pp. 764-777, (2017) (PubMed).
: "HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130811, (2016) (PubMed).
-
: "Mutations in the IFNγ-JAK-STAT pathway causing resistance to immune checkpoint inhibitors in melanoma increase sensitivity to oncolytic virus treatment." in: Clinical cancer research : an official journal of the American Association for Cancer Research, (2021) (PubMed).
-
- HLA Class I Heavy Chain
-
背景
- The HLA class I gene family is composed of a group of genes whose products encode cell surface glycoproteins of MW 40-45 kDa, associated non-covalently with the beta-2-microglobulin light chain. They include the three polymorphic molecules HLA-A, -B, and -C, which are ubiquitously expressed and which are able to present intracellular peptides to cytotoxic T cells. Three additional class I genes are known, commonly referred to as non-classical or class Ib genes, all highly homologous to the other class I genes and all of which associate with beta-2-microglobulin light chain. In humans, each of the class Ib genes appears to exhibit a distinct pattern of expression in developing and adult tissues. HLA-E transcripts are distributed widely in adult tissues and have also been found in the placenta and fetal liver. In the adult, the presence of HLA-F has been shown in skin, resting T cells, and B cells, whereas its expression during development has been reported in fetal liver and at low levels in placenta and extra-placental tissues. HLA-G was originally thought to be expressed only in certain populations of placental trophoblasts, but low levels have also been found in a variety of human tissues. Recently it was shown that HLA class I expression in breast cancer cells can have a predictive value for chemotherapy response.
抗原
-
You are here:
