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LYZ 抗体 (Biotin)

This anti-LYZ antibody is a 兔 多克隆 antibody detecting LYZ in WB, ELISA, ICC 和 IHC (fro). Suitable for 人.
产品编号 ABIN5691081
发货至: 中国

Quick Overview for LYZ 抗体 (Biotin) (ABIN5691081)

抗原

See all LYZ 抗体
LYZ (Lysozyme (LYZ))

适用

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宿主

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克隆类型

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多克隆

标记

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This LYZ antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human lysozyme.

    交叉反应 (详细)

    The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

    产品特性

    The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. In immunocytochemical and immunohistochemical techniques for the detection of lysozyme at the cellular and subcellular level in appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

    免疫原

    Highly purified recombinant human lysozyme. Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low, in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme. Freund's complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

    缓冲液

    Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

    储存液

    Without preservative

    储存条件

    RT,4 °C,-20 °C

    储存方法

    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.
  • 抗原

    LYZ (Lysozyme (LYZ))

    别名

    Lysozyme
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