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Tryptophan Hydroxylase 1 抗体

This 绵羊 多克隆 antibody specifically detects Tryptophan Hydroxylase 1 in WB 和 IHC. It exhibits reactivity toward 大鼠.
产品编号 ABIN5690709
发货至: 中国
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Quick Overview for Tryptophan Hydroxylase 1 抗体 (ABIN5690709)

抗原

See all Tryptophan Hydroxylase 1 (TPH1) 抗体
Tryptophan Hydroxylase 1 (TPH1)

适用

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  • 1
大鼠

宿主

  • 91
  • 7
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绵羊

克隆类型

  • 88
  • 17
多克隆

标记

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  • 2
  • 2
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  • 2
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  • 1
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This Tryptophan Hydroxylase 1 antibody is un-conjugated

应用范围

  • 83
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Western Blotting (WB), Immunohistochemistry (IHC)
  • 纯化方法

    Affinity Purified

    免疫原

    TPH polyclonal antibody was raised against recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E.
  • 应用备注

    Tryptophan Hydroxylase antibody can be used for specific immunolabeling of the ~55k TPH protein in Western blots of human dorsal Raphe nucleus. Applications include Dot Blots (DB) Immunohistochemistry (IHC) and Western Blots (WB). The antibody has been used successfully with rat and human brain tissues in blot immunolabeling and immunohistochemical procedures. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

    储存条件

    -20 °C

    储存方法

    For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
  • 抗原

    Tryptophan Hydroxylase 1 (TPH1)

    别名

    Tryptophan Hydroxylase

    背景

    Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis..

    分子量

    55 kDa

    基因ID

    100009100

    UniProt

    P17290

    途径

    Feeding Behaviour
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