Tryptophan Hydroxylase 1 抗体
Quick Overview for Tryptophan Hydroxylase 1 抗体 (ABIN5690709)
抗原
See all Tryptophan Hydroxylase 1 (TPH1) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
纯化方法
- Affinity Purified
-
免疫原
- TPH polyclonal antibody was raised against recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E.
-
-
-
-
应用备注
- Tryptophan Hydroxylase antibody can be used for specific immunolabeling of the ~55k TPH protein in Western blots of human dorsal Raphe nucleus. Applications include Dot Blots (DB) Immunohistochemistry (IHC) and Western Blots (WB). The antibody has been used successfully with rat and human brain tissues in blot immunolabeling and immunohistochemical procedures. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- 100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
-
储存条件
- -20 °C
-
储存方法
- For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
-
-
- Tryptophan Hydroxylase 1 (TPH1)
-
别名
- Tryptophan Hydroxylase
-
背景
- Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis..
-
分子量
- 55 kDa
-
基因ID
- 100009100
-
UniProt
- P17290
-
途径
- Feeding Behaviour
抗原
-