GM-CSF 抗体 (Biotin)
Quick Overview for GM-CSF 抗体 (Biotin) (ABIN5690566)
抗原
See all GM-CSF (CSF2) 抗体适用
宿主
克隆类型
标记
应用范围
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免疫原
- Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Rat GM-CSF specific antibody was purified by affinity chromatography and then biotinylated.
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应用备注
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ELISA:
Direct:
To detect Rat GM-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.
Sandwich
To detect Rat GM-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Rat GM-CSF (38-273) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.
Western Blot:
To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- GM-CSF
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背景
- GM-CSF is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.
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基因ID
- 116630
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UniProt
- P48750
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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