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Follistatin 抗体

This anti-Follistatin antibody is a 兔 多克隆 antibody detecting Follistatin in WB, ELISA 和 Neut. Suitable for 人.
产品编号 ABIN5690528
发货至: 中国

Quick Overview for Follistatin 抗体 (ABIN5690528)

抗原

See all Follistatin (FST) 抗体
Follistatin (FST)

适用

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宿主

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克隆类型

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多克隆

标记

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This Follistatin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix.
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100.0 ng/mL), a concentration of 8.0-12.0 μ,g/mL of this antibody is required.

    ELISA:
    Indirect:
    To detect hFollistatin by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

    Sandwich
    To detect hFollistatin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Follistatin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.

    Western Blot:
    To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    Follistatin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    Follistatin (FST)

    别名

    Follistatin

    背景

    Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. There is evidence linking polycystic ovary syndrome and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. It specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone

    基因ID

    10468

    UniProt

    P19883

    途径

    Negative Regulation of Hormone Secretion
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