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IL-15 抗体

This anti-IL-15 antibody is a 兔 多克隆 antibody detecting IL-15 in WB, ELISA 和 Neut. Suitable for 小鼠.
产品编号 ABIN5690436
发货至: 中国

Quick Overview for IL-15 抗体 (ABIN5690436)

抗原

See all IL-15 (IL15) 抗体
IL-15 (IL15) (Interleukin 15 (IL15))

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This IL-15 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-15. Murine IL-15 specific antibody was purified by affinity chromatography employing immobilized mIL-15 matrix.
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of mIL-15 (5.0 ng/mL), a concentration of 0.4 - 0.5 μ,g/mL of this antibody is required.

    ELISA:
    Indirect:
    To detect mIL-15 by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.

    Sandwich
    To detect mIL-15 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-15 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-15.

    Western Blot:
    To detect mIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    IL-15 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    IL-15 (IL15) (Interleukin 15 (IL15))

    别名

    IL-15

    背景

    IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively.

    基因ID

    16168

    UniProt

    P48346

    途径

    JAK/STAT Signaling, Glycosaminoglycan Metabolic Process
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