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IL-33 抗体

IL33 适用: 人 WB, ELISA, IHC, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5690010
发货至: 中国
  • 抗原 See all IL-33 (IL33) 抗体
    IL-33 (IL33) (Interleukin 33 (IL33))
    适用
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    宿主
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    克隆类型
    • 111
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    多克隆
    标记
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    This IL-33 antibody is un-conjugated
    应用范围
    • 84
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut)
    纯化方法
    IL-33 specific antibody was purified by affinity chromatography employing immobilized hIL-33 matrix.
    免疫原
    E.coli derived Recombinant Human IL-33
    Top Product
    Discover our top product IL33 Primary Antibody
  • 应用备注
    ELISA:
    Sandwich:
    To detect hIL-33 by sandwich ELISA (using 100μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Polyclonal Biotinylated Anti-Human IL-33 (38-193) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33.

    Western Blot:

    To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Neutralization:

    To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/mL), a concentration of 0.2-0.4 μ,g/mL of this antibody is required.

    Immunohistochemistry:

    This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 mg/mL-0.25 mg/mL with an overnight incubation at 4˚,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6.0 sodium citrate buffer is recommended.
    限制
    仅限研究用
  • 状态
    Lyophilized
    储存条件
    -20 °C
    储存方法
    The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原
    IL-33 (IL33) (Interleukin 33 (IL33))
    别名
    IL-33 (IL33 产品)
    别名
    IL33 antibody, C9orf26 antibody, DVS27 antibody, IL1F11 antibody, NF-HEV antibody, NFEHEV antibody, RP11-575C20.2 antibody, 9230117N10Rik antibody, Il-33 antibody, Il1f11 antibody, RGD1311155 antibody, interleukin 33 antibody, IL33 antibody, Il33 antibody
    基因ID
    90865
    UniProt
    O95760
    途径
    Production of Molecular Mediator of Immune Response
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