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IL-33 抗体

This anti-IL-33 antibody is a 兔 多克隆 antibody detecting IL-33 in WB, ELISA, IHC 和 Neut. Suitable for 人.
产品编号 ABIN5690010
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL-33 抗体 (ABIN5690010)

抗原

See all IL-33 (IL33) 抗体
IL-33 (IL33) (Interleukin 33 (IL33))

适用

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宿主

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克隆类型

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多克隆

标记

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This IL-33 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut)
  • 纯化方法

    IL-33 specific antibody was purified by affinity chromatography employing immobilized hIL-33 matrix.

    免疫原

    E.coli derived Recombinant Human IL-33
  • 应用备注

    ELISA:
    Sandwich:
    To detect hIL-33 by sandwich ELISA (using 100μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Polyclonal Biotinylated Anti-Human IL-33 (38-193) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33.

    Western Blot:

    To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Neutralization:

    To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/mL), a concentration of 0.2-0.4 μ,g/mL of this antibody is required.

    Immunohistochemistry:

    This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 mg/mL-0.25 mg/mL with an overnight incubation at 4˚,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6.0 sodium citrate buffer is recommended.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    IL-33 (IL33) (Interleukin 33 (IL33))

    别名

    IL-33

    基因ID

    90865

    UniProt

    O95760

    途径

    Production of Molecular Mediator of Immune Response
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