IL-33 抗体
Quick Overview for IL-33 抗体 (ABIN5690010)
抗原
See all IL-33 (IL33) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
纯化方法
- IL-33 specific antibody was purified by affinity chromatography employing immobilized hIL-33 matrix.
-
免疫原
- E.coli derived Recombinant Human IL-33
-
-
anti-Interleukin 33 (IL33) (AA 112-270) antibody
IL33 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Interleukin 33 (IL33) antibodyIL33 适用: 人 WB, ELISA, IHC, IP, Func 宿主: 小鼠 Monoclonal IL33305B unconjugated
anti-Interleukin 33 (IL33) (AA 112-270) antibodyIL33 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C19 unconjugated
anti-Interleukin 33 (IL33) (AA 110-261) antibodyIL33 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 2# unconjugated
anti-Interleukin 33 (IL33) (AA 185-264) antibodyIL33 适用: 小鼠, 大鼠 ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) antibodyIL33 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) (AA 171-269) antibodyIL33 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Interleukin 33 (IL33) (AA 102-264) antibodyIL33 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) antibodyIL33 适用: 人 WB, IHC (p), EIA, Func 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) antibody (Biotin)IL33 适用: 人 WB, EIA 宿主: 兔 Polyclonal Biotin
-
-
应用备注
-
ELISA:
Sandwich:
To detect hIL-33 by sandwich ELISA (using 100μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Polyclonal Biotinylated Anti-Human IL-33 (38-193) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-33.
Western Blot:
To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-33 (1.0 ng/mL), a concentration of 0.2-0.4 μ,g/mL of this antibody is required.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 mg/mL-0.25 mg/mL with an overnight incubation at 4˚,C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
储存条件
- -20 °C
-
储存方法
- The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
-
-
- IL-33 (IL33) (Interleukin 33 (IL33))
-
别名
- IL-33
-
基因ID
- 90865
-
UniProt
- O95760
-
途径
- Production of Molecular Mediator of Immune Response
抗原
-
