Artemin antibody was purified by affinity chromatography and then biotinylated.
免疫原
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hArtemin. Human Artemin specific antibody was purified by affinity chromatography and then biotinylated.
Direct: To detect hArtemin by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin. Sandwich: To detect hArtemin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required.
Western Blot: To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
限制
仅限研究用
状态
Lyophilized
储存条件
-20 °C
储存方法
Artemin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
抗原
ARTN
(Artemin (ARTN))
别名
Artemin
背景
Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons.