This antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177.
纯化方法
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
免疫原
Bcr (Phospho-Tyr177) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 177 (P-F-Y (p) -V-N) derived from Human Bcr.
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store antibody at -20°C for up to one year.
抗原
BCR
(Breakpoint Cluster Region (BCR))
别名
Bcr
背景
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.