CNTF 抗体
Quick Overview for CNTF 抗体 (ABIN5689201)
抗原
See all CNTF 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-rCNTF specific antibody was purified by affinity chromatography employing immobilized rCNTF matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rCNTF (Rat Ciliary Neurotrophic Factor).
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anti-Ciliary Neurotrophic Factor (CNTF) (C-Term) antibody
CNTF 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 1-200) antibodyCNTF 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-198) antibodyCNTF 适用: 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-200) antibodyCNTF 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-200) antibodyCNTF 适用: 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 21-150) antibodyCNTF 适用: 人, 大鼠, 兔 WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-198) antibodyCNTF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 2-200) antibodyCNTF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 4-196) antibodyCNTF 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor (CNTF) (AA 1-200) antibodyCNTF 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/mL), a concentration of 0.02 - 0.05 μ,g/mL of this antibody is required.
ELISA:
To detect rCNTF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF.
Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.
Western Blot:
To detect rCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- CNTF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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别名
- CNTF
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背景
- Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.
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基因ID
- 25707
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UniProt
- P20294
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途径
- JAK/STAT Signaling
抗原
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