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CCL5 抗体 (Biotin)

This anti-CCL5 antibody is a 兔 多克隆 antibody detecting CCL5 in WB 和 ELISA. Suitable for 大鼠.
产品编号 ABIN5689197
发货至: 中国

Quick Overview for CCL5 抗体 (Biotin) (ABIN5689197)

抗原

See all CCL5 抗体
CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This CCL5 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA
  • 纯化方法

    Anti-rat RANTES specific antibody was purified by affinity chromatography and then biotinylated.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat RANTES (rat RANTES).
  • 应用备注

    ELISA:

    To detect rat RANTES by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat RANTES.

    Sandwich:

    To detect Rat RANTES by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat RANTES (XP-5258) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat RANTES.

    Western Blot:

    To detect rat RANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat RANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    RANTES antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

    别名

    RANTES

    背景

    RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

    基因ID

    81780

    UniProt

    P50231

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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