Ccl12 抗体
Quick Overview for Ccl12 抗体 (ABIN5689163)
抗原
See all Ccl12 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-mMCP-5 specific antibody was purified by affinity chromatography employing immobilized mMCP-5 matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-5 (murine MCP-5).
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anti-Chemokine (C-C Motif) Ligand 12 (Ccl12) antibody
Ccl12 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 12 (Ccl12) antibody (Biotin)Ccl12 适用: 小鼠 ELISA, WB, Neut 宿主: 兔 Polyclonal Biotin
anti-Chemokine (C-C Motif) Ligand 12 (Ccl12) (AA 28-104) antibodyCcl12 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMCP-5 (100 ng/mL), a concentration of 2.0 - 3.0 μ,g/mL of this antibody is required.
ELISA:
To detect mMCP-5 by direct ELISA (using 100 μ,g/mL of this μ,L/well antibody solution) a concentration of at least 0.5 antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMCP-5.
Sandwich:
To detect mMCP-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MCP-5 (XP-5226Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.
Western Blot:
To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- MCP-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
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别名
- MCP-5
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背景
- MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations
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基因ID
- 20293
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UniProt
- Q62401
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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