Ccl12 抗体 (Biotin)
Quick Overview for Ccl12 抗体 (Biotin) (ABIN5689162)
抗原
See all Ccl12 抗体适用
宿主
克隆类型
标记
应用范围
-
-
纯化方法
- Anti-mMCP-5 specific antibody was purified by affinity chromatography and then biotinylated.
-
免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-5 (murine MCP-5).
-
-
-
-
应用备注
-
ELISA:
To detect mMCP-5 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mMCP-5.
Sandwich:
To detect mMCP-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MCP-5 (XP-5226) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-5.
Western Blot:
To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
储存条件
- -20 °C
-
储存方法
- MCP-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
-
-
- Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))
-
别名
- MCP-5
-
背景
- MCP-5 is a member of the b-chemokine family (C-C) of cytokines. This chemokine is strongly chemotactic for human monocytes and murine peritoneal macrophages, but is not active on mouse neutrophils and shows only a weak response on mouse eosinophils at high concentrations
-
基因ID
- 20293
-
UniProt
- Q62401
-
途径
- Cellular Response to Molecule of Bacterial Origin
抗原
-