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CXCL10 抗体

This anti-CXCL10 antibody is a 兔 多克隆 antibody detecting CXCL10 in WB, ELISA 和 Neut. Suitable for 小鼠.
产品编号 ABIN5689154
发货至: 中国

Quick Overview for CXCL10 抗体 (ABIN5689154)

抗原

See all CXCL10 抗体
CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This CXCL10 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-mIP-10 specific antibody was purified by affinity chromatography employing immobilized mIP-10 matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIP-10 (murine IP-10).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of mIP-10 (15.00 ng/mL), a concentration of 0.5 0.9 μ,g/mL of this antibody is required.

    ELISA:

    To detect mIP-10 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIP-10.

    Sandwich:
    br>To detect mIP-10 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IP-10 (XP-5204Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.

    Western Blot:

    To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ng/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    IP-10 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))

    别名

    IP-10

    背景

    Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.

    基因ID

    15945

    UniProt

    P17515
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