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IL-9 抗体

This anti-IL-9 antibody is a 兔 多克隆 antibody detecting IL-9 in WB, ELISA 和 Neut. Suitable for 小鼠.
产品编号 ABIN5689152
发货至: 中国

Quick Overview for IL-9 抗体 (ABIN5689152)

抗原

See all IL-9 (IL9) 抗体
IL-9 (IL9) (Interleukin 9 (IL9))

适用

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小鼠

宿主

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克隆类型

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多克隆

标记

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This IL-9 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-mIL-9 specific antibody was purified by affinity chromatography employing immobilized mIL-9 matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-9 (murine IL-9).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of mIL-9 (0.30 ng/mL), a concentration of 0.006 - 0.012 μ,g/mL of this antibody is required.

    ELISA:

    To detect mIL-9 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-9.

    Sandwich:

    To detect mIL-9 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-9 (XP-5201Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-9.

    Western Blot:
    To detect mIL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    IL-9 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    IL-9 (IL9) (Interleukin 9 (IL9))

    别名

    IL-9

    背景

    IL-9 is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors, but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL-9 stimulates cell proliferation and prevents apoptosis. The IL-9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL-9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma

    基因ID

    16198

    UniProt

    P15247

    途径

    JAK/STAT Signaling
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