Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mExodus-2 (100.0 ng/mL), a concentration of 9.0-10.0 μ,g/mL of this antibody is required.
ELISA:
To detect mExodus-2 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mExodus-2.
Sandwich: To detect mExodus-2 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Exodus-2 (XP-5129Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mExodus-2.
Western Blot:
To detect mExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mExodus-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
限制
仅限研究用
状态
Lyophilized
储存条件
-20 °C
储存方法
Exodus-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
抗原
CCL21
(Chemokine (C-C Motif) Ligand 21 (CCL21))
别名
Exodus-2
背景
Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils.