CCL5 抗体
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北京 101111
Quick Overview for CCL5 抗体 (ABIN5689104)
抗原
See all CCL5 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hRANTES specific antibody was purified by affinity chromatography employing immobilized hRANTES matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hRANTES (human RANTES).
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100 ng/mL), a concentration of 5.0 - 7.0 μ,g/mL of this antibody is required.
ELISA:
To detect hRANTES by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hRANTES.
Sandwich:
To detect hRANTES by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human RANTES (XP-5257Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- RANTES antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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别名
- RANTES
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背景
- RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
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基因ID
- 6352
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UniProt
- P13501
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
抗原
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