CXCL10 抗体
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北京 101111
Quick Overview for CXCL10 抗体 (ABIN5689055)
抗原
See all CXCL10 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hIP-10 specific antibody was purified by affinity chromatography employing immobilized hIP-10 matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIP-10 (human IP-10).
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIP-10 (100.0 ng/mL), a concentration of 2.0 - 3.0 μ,g/mL of this antibody is required.
ELISA:
To detect hIP-10 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIP-10.
Sandwich:
To detect hIP-10 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IP-10 (XP-5203Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIP-10.
Western Blot:
To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- IP-10 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
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别名
- IP-10
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背景
- Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.
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基因ID
- 3627
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UniProt
- P02778
抗原
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