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IL-5 抗体

IL5 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5689047
发货至: 中国
  • 抗原 See all IL-5 (IL5) 抗体
    IL-5 (IL5) (Interleukin 5 (IL5))
    适用
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    宿主
    • 49
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    克隆类型
    • 50
    • 46
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    多克隆
    标记
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    This IL-5 antibody is un-conjugated
    应用范围
    • 62
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    纯化方法
    Anti-hIL-5 specific antibody was purified by affinity chromatography employing immobilized hIL-5 matrix.
    免疫原
    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-5 (human Interleukin-5).
    Top Product
    Discover our top product IL5 Primary Antibody
  • 应用备注
    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/mL), a concentration of 0.05 -0.09 μ,g/mL of this antibody is required.

    ELISA:

    To detect hIL-5 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.

    Sandwich:

    To detect hIL-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-5 (XP-5191Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5.

    Western Blot:

    To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    限制
    仅限研究用
  • 状态
    Lyophilized
    储存条件
    -20 °C
    储存方法
    IL-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原
    IL-5 (IL5) (Interleukin 5 (IL5))
    别名
    IL-5 (IL5 产品)
    别名
    EDF antibody, IL-5 antibody, TRF antibody, Il-5 antibody, IL5 antibody, interleukin 5 antibody, IL5 antibody, Il5 antibody
    背景
    Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL-2, IL-4 and growth hormone. Human and mouse IL-5 have 70 % amino acid sequence homology.
    基因ID
    3567
    UniProt
    P05113
    途径
    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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