IL-5 抗体
Quick Overview for IL-5 抗体 (ABIN5689047)
抗原
See all IL-5 (IL5) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hIL-5 specific antibody was purified by affinity chromatography employing immobilized hIL-5 matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-5 (human Interleukin-5).
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anti-Interleukin 5 (IL5) (AA 20-134) antibody
IL5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 21-70) antibodyIL5 适用: 人, 小鼠, 大鼠, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 56-132) antibodyIL5 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 人 FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 人 WB 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 山羊 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 人 ELISA, D, ELISA (Capture) 宿主: 大鼠 Monoclonal JES1-39D10 unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 小鼠 ELISA 宿主: 大鼠 Monoclonal TRFK5 unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5 (0.45 ng/mL), a concentration of 0.05 -0.09 μ,g/mL of this antibody is required.
ELISA:
To detect hIL-5 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-5.
Sandwich:
To detect hIL-5 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-5 (XP-5191Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:
To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- IL-5 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- IL-5 (IL5) (Interleukin 5 (IL5))
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别名
- IL-5
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背景
- Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL-2, IL-4 and growth hormone. Human and mouse IL-5 have 70 % amino acid sequence homology.
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基因ID
- 3567
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UniProt
- P05113
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
抗原
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