IL16 抗体
Quick Overview for IL16 抗体 (ABIN5689034)
抗原
See all IL16 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Anti-hIL-16 specific antibody was purified by affinity chromatography employing immobilized hIL-16 matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-16 (human Interleukin-16).
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anti-Interleukin 16 (IL16) (AA 1203-1332) antibody
IL16 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 16 (IL16) (AA 1212-1332) antibodyIL16 适用: 人 WB, ELISA, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 16 (IL16) (AA 1203-1322) antibodyIL16 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 16 (IL16) (C-Term) antibodyIL16 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 16 (IL16) (AA 1-454) antibodyIL16 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 16 (IL16) (C-Term) antibodyIL16 适用: 人, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 16 (IL16) antibodyIL16 适用: 人 IHC, IHC (p) 宿主: 小鼠 Monoclonal G11-II unconjugated
anti-Interleukin 16 (IL16) antibody (Biotin)IL16 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 16 (IL16) (AA 1212-1332) antibodyIL16 适用: 人 WB, ELISA, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 16 (IL16) (AA 900-960) antibodyIL16 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.20 ng/mL), a concentration of 0.12 - 0.15 μ,g/mL of this antibody is required.
ELISA:
To detect hIL-16 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16.
Sandwich:
To detect hIL-16 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-16 (XP-5170Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-16.
Western Blot:
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- IL-16 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- IL16 (Interleukin 16 (IL16))
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别名
- IL-16
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背景
- IL16 is a proinflammatory cytokine that is chemotactic for CD4+ T lymphocytes, monocytes, and eosinophils. IL16 is synthesized as a precursor molecule (pro IL16) of approximately 68 kDa and 631 amino acid residues lacking a signal peptide. The sequence and structure of IL16 is conserved across species. In addition to inducing chemotaxis, IL16 upregulates the IL2 receptor and upregulates HLADR expression. It also inhibits T cell receptor (TCR)/CD3 dependent activation, and suppresses HIV1 replication in vitro. Sources of IL16 include epithelial cells, mast cells, T lymphocytes (CD4+ and CD8+), macrophages, synovial fibroblasts, and eosinophils.
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基因ID
- 3603
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UniProt
- Q9UME6
抗原
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