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IGF2 抗体

This anti-IGF2 antibody is a 兔 多克隆 antibody detecting IGF2 in WB, ELISA 和 Neut. Suitable for 人.
产品编号 ABIN5689024
发货至: 中国

Quick Overview for IGF2 抗体 (ABIN5689024)

抗原

See all IGF2 抗体
IGF2 (Insulin-Like Growth Factor 2 (IGF2))

适用

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宿主

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克隆类型

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多克隆

标记

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This IGF2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-hIGF-II specific antibody was purified by affinity chromatography employing immobilized hIGF-II matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-II (human Insulin Like Growth Factor-II).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1 4.6 mg/mL of this antibody is required.

    ELISA:

    To detect hIGF-II by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-II.

    Sandwich:

    To detect hIGF-II by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-II (XP-5160Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.

    Western Blot:

    To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    IGF-II antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    IGF2 (Insulin-Like Growth Factor 2 (IGF2))

    别名

    IGF-II

    基因ID

    3481

    UniProt

    P01344

    途径

    Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation
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