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FGF acidic 抗体

The 兔 多克隆 anti-FGF acidic antibody (ABIN5688992) specifically detects FGF acidic in ELISA, WB 和 Neut. The antibody is reactive with 人 samples.
产品编号 ABIN5688992
发货至: 中国
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Quick Overview for FGF acidic 抗体 (ABIN5688992)

抗原

FGF acidic

适用

宿主

  • 2
  • 1

克隆类型

  • 2
  • 1
多克隆

标记

  • 2
  • 1
非结合性

应用范围

ELISA, Western Blotting (WB), Neutralization (Neut)
  • 纯化方法

    Anti-hFGF-acidic specific antibody was purified by affinity chromatography employing immobilized hFGF-acidic matrix.

    免疫原

    Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-acidic (human Fibroblast Growth Factor-acidic).
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-acidic (30.0 ng/mL), a concentration of 0.46 - 0.9 μ,g/mL of this antibody is required.

    ELISA:

    To detect hFGF-acidic by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-acidic.

    Sandwich: To detect hFGF-acidic by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-acidic (XP-5136Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-acidic.

    Western Blot:

    To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    FGF-acidic antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    FGF acidic

    别名

    FGF-acidic

    基因ID

    2246

    UniProt

    P05230
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