FGF acidic 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in ELISA, WB 和 Neut. Suitable for 人.
Quick Overview for FGF acidic 抗体 (ABIN5688992)
抗原
FGF acidic
适用
人
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB), Neutralization (Neut)
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纯化方法
- Anti-hFGF-acidic specific antibody was purified by affinity chromatography employing immobilized hFGF-acidic matrix.
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免疫原
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-acidic (human Fibroblast Growth Factor-acidic).
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应用备注
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-acidic (30.0 ng/mL), a concentration of 0.46 - 0.9 μ,g/mL of this antibody is required.
ELISA:
To detect hFGF-acidic by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-acidic.
Sandwich: To detect hFGF-acidic by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-acidic (XP-5136Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-acidic.
Western Blot:
To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- FGF-acidic antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- FGF acidic
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别名
- FGF-acidic
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基因ID
- 2246
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UniProt
- P05230
抗原
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