Synaptotagmin antibody is specific for Mr 60 - 62k synaptotagmin phosphorylated at Ser309.
纯化方法
Affinity Purified
免疫原
Synaptotagmin (Ser309) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Ser309 of synaptotagmin.
SYT
适用: 大鼠, 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 680
应用备注
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, and rat have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
限制
仅限研究用
状态
Liquid
缓冲液
100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
储存条件
-20 °C
储存方法
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
抗原
Synaptotagmin (SYT)
别名
Synaptotagmin
背景
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis. Moreover recent studies indicate that the protein also plays a key role in endocytosis. Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin ability to influence both the exocytotic and endocytotic components of synaptic transmission.