MARCKS 抗体 (pSer152, pSer156)
Quick Overview for MARCKS 抗体 (pSer152, pSer156) (ABIN5688889)
抗原
See all MARCKS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer152, pSer156
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特异性
- MARCKS antibody is specific for MARCKS phosphorylated at Ser152 and Ser156.
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纯化方法
- Affinity Purified
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免疫原
- MARCKS (Ser152/156) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152 and phospho-Ser156 of rat MARCKS.
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-65) antibody
MARCKS 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 1-100) antibodyMARCKS 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer170) antibodyMARCKS 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer158) antibodyMARCKS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer162) antibodyMARCKS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 2-66) antibodyMARCKS 适用: 人, 小鼠 WB, ELISA, IF, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (Internal Region) antibodyMARCKS 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer152), (Ser156) antibodyMARCKS 适用: 人, 小鼠, 大鼠, Cow, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (AA 31-120) antibodyMARCKS 适用: 大鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) (pSer159) antibodyMARCKS 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Immunolabeling of the 87 kDa band in rat brain homogenate is blocked by the Ser152/156 phosphopeptide but not by the non-phosphopeptide. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) not yet determined. Human, mouse, rat, and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 100 μLin 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.
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储存条件
- -20 °C
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储存方法
- For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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别名
- MARCKS
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背景
- Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Moreover, MARCKS is a member of a family of calmodulin binding proteins. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.
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分子量
- 87 kDa
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基因ID
- 25603
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UniProt
- P30009
抗原
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