CCL26
适用: 人
WB, FACS, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 680
应用备注
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEotaxin-3 (2.0 ng/mL), a concentration of 1.0-5.0 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 1000 pg/mL of recombinant human Eotaxin-3 when used with our biotinylated antigen affinity purified anti-human Eotaxin-3 as the detection antibody at a concentration of approximately 0.25-0.50 μ,g/mL.
Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.
限制
仅限研究用
状态
Lyophilized
储存条件
4 °C,-20 °C
储存方法
Eotaxin-3 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.