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CCL8 抗体

This 小鼠 单克隆 anti-CCL8 antibody (Clone 1-1_2D4-1A3) specifically detects CCL8 in WB, ELISA 和 Neut. The antibody is reactive with 人 samples.
产品编号 ABIN5688696
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CCL8 抗体 (ABIN5688696)

抗原

See all CCL8 抗体
CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CCL8 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)

克隆位点

1-1_2D4-1A3
  • 纯化方法

    The monoclonal antibody against human MCP-2 designated as VL-1 was purified from ascites fluid on a Protein A affinity column.

    免疫原

    The monoclonal antibody was derived form BALB/c X ICR F1 mice and belongs to the IgG1K isotype.
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL), a concentration of 0.8-1.0 μ,g/mL of this antibody is required.

    ELISA:

    In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

    Western Blot:

    To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    4 °C,-20 °C

    储存方法

    MCP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
  • 抗原

    CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

    别名

    MCP-2

    背景

    MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.

    基因ID

    6355

    UniProt

    P80075
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