CCL8
适用: 人
IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 750
应用备注
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL), a concentration of 0.8-1.0 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.
Western Blot:
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions.
限制
仅限研究用
状态
Lyophilized
储存条件
4 °C,-20 °C
储存方法
MCP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
抗原
CCL8
(Chemokine (C-C Motif) Ligand 8 (CCL8))
别名
MCP-2
背景
MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.