CCL8 抗体
Our Local Distributor
北京 101111
Quick Overview for CCL8 抗体 (ABIN5688696)
抗原
See all CCL8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
纯化方法
- The monoclonal antibody against human MCP-2 designated as VL-1 was purified from ascites fluid on a Protein A affinity column.
-
免疫原
- The monoclonal antibody was derived form BALB/c X ICR F1 mice and belongs to the IgG1K isotype.
-
-
-
-
应用备注
-
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100 ng/mL), a concentration of 0.8-1.0 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 1.0-2.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-2 when used with our biotinylated antigen affinity purified anti-human MCP-2 (XP-5224Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.
Western Blot:
To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, under non-reducing conditions. -
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
储存条件
- 4 °C,-20 °C
-
储存方法
- MCP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
-
-
- CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))
-
别名
- MCP-2
-
背景
- MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.
-
基因ID
- 6355
-
UniProt
- P80075
抗原
-