Adipolean 抗体
This 山羊 多克隆 anti-not_set antibody specifically detects not_set in ELISA 和 WB. The antibody is reactive with 人 samples.
产品编号 ABIN5688337
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for Adipolean 抗体 (ABIN5688337)
抗原
Adipolean
适用
人
宿主
山羊
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB)
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免疫原
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hgAcrp30/Adipolean (human gAcrp30/Adipolean).
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应用备注
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ELISA:
To detect hgAcrp30/Adipolean by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hgAcrp30/Adipolean.
Western Blot:
To detect hgAcrp30/Adipolean by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hgAcrp30/Adipolean is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
限制
- 仅限研究用
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状态
- Lyophilized
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储存条件
- -20 °C
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储存方法
- Adipolean antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Adipolean
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背景
- Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
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基因ID
- 9370
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UniProt
- Q15848
抗原
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