The immunizing peptide represents part of the extracellular domain.
纯化方法
OSMR Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
OSMR Antibody was raised against synthetic peptide QIHGEQLDPHVT found at an internal region of OSMR.
OSM
适用: 大鼠
ELISA, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 125 kDa band observed in lysates of cell lines HeLa, A549 and U937 (calculated MW of 111 kDa according to NP_003990.1). Recommended concentration: 0.1-0.3μ,g/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
OSMR is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot and store at -20°C. Minimize freezing and thawing.