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Thrombopoietin 抗体

This 山羊 多克隆 antibody specifically detects Thrombopoietin in WB, ELISA 和 Neut. It exhibits reactivity toward 人.
产品编号 ABIN5687619
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Thrombopoietin 抗体 (ABIN5687619)

抗原

See all Thrombopoietin (THPO) 抗体
Thrombopoietin (THPO)

适用

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宿主

  • 38
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山羊

克隆类型

  • 50
  • 14
多克隆

标记

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This Thrombopoietin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 纯化方法

    Anti-hTPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.

    免疫原

    Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO.
  • 应用备注

    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5 ng/mL), a concentration of 4.2 - 6.3 μ,g/mL of this antibody is required.

    ELISA:

    To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTPO.

    Sandwich:

    To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TPO (XP-5288Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

    Western Blot:
    To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    Thrombopoietin (THPO)

    别名

    Thrombopoietin (TPO)

    背景

    Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

    基因ID

    7066

    UniProt

    P40225

    途径

    JAK/STAT Signaling, Hormone Activity
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