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Thrombopoietin 抗体 (Biotin)

The Biotin-conjugated 山羊 多克隆 anti-Thrombopoietin antibody (ABIN5687618) specifically detects Thrombopoietin in WB 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN5687618
发货至: 中国
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北京 101111
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Quick Overview for Thrombopoietin 抗体 (Biotin) (ABIN5687618)

抗原

See all Thrombopoietin (THPO) 抗体
Thrombopoietin (THPO)

适用

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宿主

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山羊

克隆类型

  • 50
  • 14
多克隆

标记

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This Thrombopoietin antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA
  • 纯化方法

    Anti-hTPO specific antibody was purified by affinity chromatography and then biotinylated.

    免疫原

    Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO (human TPO).
  • 应用备注

    ELISA:

    To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hTPO.

    Sandwich:

    To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TPO (XP-5288) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

    Western Blot:
    To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    限制

    仅限研究用
  • 状态

    Lyophilized

    储存条件

    -20 °C

    储存方法

    TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • 抗原

    Thrombopoietin (THPO)

    别名

    Thrombopoietin (TPO)

    背景

    Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

    基因ID

    7066

    UniProt

    P40225

    途径

    JAK/STAT Signaling, Hormone Activity
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