CCL26
适用: 人
WB, FACS, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 680
应用备注
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human eotaxin-3 (100.00 ng/mL), a concentration of 5.0 - 7.0 μ,g/mL of this antibody is required.
ELISA:
To detect human eotaxin-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human eotaxin-3.
Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Eotaxin-3 (XP-5127Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.
Western Blot:
To detect human eotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant human eotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
限制
仅限研究用
状态
Lyophilized
储存条件
-20 °C
储存方法
Eotaxin-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.