Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
特异性
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
产品特性
Molar Ratio: Biotin/IgG ~5.2
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
Uridine-5’-Diphosphoglucose Dehydrogenase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
溶解方式
Restore by adding 1.0 mL of sterile distilled water
浓度
10.0 mg/mL
缓冲液
PBS, pH 7.2 without preservatives and foreign proteins
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.Synonyms: UDP-Glc dehydrogenase, UDP-GlcDH, UDP-glucose 6-dehydrogenase, UDPGDH