Ficin 抗体 (Biotin)
This anti-Ficin antibody is a 兔 多克隆 antibody detecting Ficin in IF, WB, DB, EIA, ID, IP 和 RIA. Suitable for Fig.
Quick Overview for Ficin 抗体 (Biotin) (ABIN568456)
抗原
Ficin
适用
Fig
宿主
兔
克隆类型
多克隆
标记
This Ficin antibody is conjugated to Biotin
应用范围
Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
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产品特性
- Molar Ratio: Biotin/IgG ~ 6.0
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纯化方法
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
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免疫原
- Ficin isolated and purified from fig tree latex. Freund’s complete adjuvant is used in the first step of the immunization procedure
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亚型
- IgG
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore by adding 1.0 mL of sterile distilled water
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浓度
- 10.0 mg/mL
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缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Ficin
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背景
- Ficin is classified as a Thiol Protease. It contains a single reactive Cysteine at its active site. The amino acid homology of the active site is similar to that of Papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. (3) Ficin is inhibited by Iodoacetamide, Iodoacetic Acid, N-Ethylmaleimide, Mercuric Chloride, DFP (diisopropyl fluorophosphate), TLCK (N-alpha-p-Tosyl-lysine Chloromethyl ketone), and TPCK (N-Tosyl-L-Phenylalanine Chloromethyl Ketone). (1,2) Enzyme Commission (EC) Number: 3.4.22.3 Molecular Weight: 23.8 kDa (1). Extinction Coefficient: E1 % = 21.0 (280 nm) (2)
抗原
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