Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
特异性
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to succinic thiokinase from porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
Succinic thiokinase isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.