Subtilisin 抗体
The 兔 多克隆 anti-Subtilisin antibody is suitable to detect Subtilisin in samples from Bacillus. It has been validated for IF, WB, DB, EIA, ID, IP 和 RIA.
产品编号 ABIN568427
发货至:
中国
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中国
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Quick Overview for Subtilisin 抗体 (ABIN568427)
抗原
Subtilisin
适用
Bacillus
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Immunofluorescence (IF), Western Blotting (WB), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (RIA)
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特异性
- Subtilisin from Bacillus subtilis. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
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纯化方法
- Solid Phase Affinity Chromatography
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免疫原
- Subtilisin isolated and purified from Bacillus subtilis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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溶解方式
- Restore by adding 0.5 mL of sterile distilled water
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浓度
- 2.0 mg/mL
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缓冲液
- PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Subtilisin
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背景
- Subtilisin (serine endopeptidase) is a non-specific protease (a protein-digesting enzyme) initially obtained from Bacillus subtilis. Subtilisins belong to subtilases, a group of serine proteases that initiate the nucleophilic attack on the peptide (amide) bond through a serine residue at the active site. They are physically and chemically well-characterized enzymes. Subtilisins typically have molecular weights of about 20,000 to 45,000 dalton. They can be obtained from soil bacteria, for example, Bacillus amyloliquefaciens. Subtilisins are secreted in large amounts from many Bacillus species. Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.Synonyms: Subtilisin E, apr, aprA, aprE, sprE
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基因ID
- 939313
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NCBI登录号
- NP_388911
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UniProt
- P04189
抗原
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