cFBPase (Cytosolic) 抗体
(7 references)Our Local Distributor
北京 101111
Quick Overview for cFBPase (Cytosolic) 抗体 (ABIN5684015)
抗原
适用
宿主
克隆类型
应用范围
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抗原表位
- Cytosolic
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原理
- Anti-cFBPase | Cytosolic fructose-1,6-bisphosphatase (cytoplasm marker in photosynthetic tissues)
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交叉反应 （详细）
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Arabidopsis thaliana, Brassica napus, Macroptilium atropurpureum, Nicotiana benthamiana, Pinus silvestris, Pinus yunanniensis, Oryza sativa, Petunia hybrida cv. Mitchell, Solanum tuberosum, Zea mays
Not reactive in: Chlamydomonas reinhardtii -
预测反应
- Capsella rubella,Pisum sativum,Ricinus communis,Glycine max,Phaseolus vulgaris,Sesamum indicum,Spinacia oleracea,Populus trichocarpa,Vitis vinifera
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纯化方法
- Serum
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免疫原
- Overexpressed cytosolic fructose 1
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应用备注
- 1: 500 (IL), 1 : 5 000 (WB)
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说明
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for image of detection please refer to Strand et al. 2000. This antibody does not react with chloroplastic form of FBPase.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).
: "Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodeling in the Arabidopsis response to phosphate starvation." in: Journal of proteomics, Vol. 94C, pp. 1-22, (2013) (PubMed).
: "Arabidopsis Deficient in Cutin Ferulate encodes a transferase required for feruloylation of ω-hydroxy fatty acids in cutin polyester." in: Plant physiology, Vol. 158, Issue 2, pp. 654-65, (2012) (PubMed).
: "Signal transduction by IRE1-mediated splicing of bZIP50 and other stress sensors in the endoplasmic reticulum stress response of rice." in: The Plant journal : for cell and molecular biology, Vol. 69, Issue 6, pp. 946-56, (2012) (PubMed).
: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).
: "Isobaric tags for relative and absolute quantification- based comparative proteomics reveals the features of plasma membrane-associated proteomes of pollen grains and pollen tubes from Lilium davidii." in: Journal of integrative plant biology, Vol. 52, Issue 12, pp. 1043-58, (2010) (PubMed).
: "Decreased expression of two key enzymes in the sucrose biosynthesis pathway, cytosolic fructose-1,6-bisphosphatase and sucrose phosphate synthase, has remarkably different consequences for photosynthetic carbon metabolism in transgenic Arabidopsis thalian" in: The Plant journal : for cell and molecular biology, Vol. 23, Issue 6, pp. 759-70, (2000) (PubMed).
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: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).
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- cFBPase
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背景
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Background: Fructose-1,6 bisphosphatase (FBPase) (EC=3.1.311) is one of the regulatory enzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1,6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours. Alternative name: D-fructose-1,6-bisphosphate 1-phosphohydrolase
Additional information: Kinetic and allosteric properties of the plant cytosolic FBPase are remarkably similar to the mammalian and yeast FBPase, but differ greatly from those of the chloroplastic FBPase. The antibody could detect FBPase from the human COS-7 cell line transfected with FBP1 expressing vector.This product can be sold containing ProClin if requested.
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分子量
- 45 | 37 kDa (Arabidopsis thaliana)
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基因ID
- 840953
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UniProt
- Q9MA79
抗原
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