cFBPase (Cytoplasmic) 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in WB. Suitable for Arabidopsis thaliana, Oryza sativa 和 Zea mays subsp. parviglumis (Balsas teosinte). This Primary Antibody has been cited in 7+ publications.
(7 references)Quick Overview for cFBPase (Cytoplasmic) 抗体 (ABIN5684015)
抗原
cFBPase
适用
Arabidopsis thaliana, Oryza sativa, Zea mays subsp. parviglumis (Balsas teosinte)
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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抗原表位
- Cytoplasmic
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交叉反应 （详细）
- No cross-reactivity with: Chlamydomonas reinhardtii
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 45 / 37 kDa (Arabidopsis thaliana)
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纯化方法
- serum
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免疫原
- overexpressed cytosolic fructose 1,6 bisphosphatase (cFBPase) derived from the sequence from Arabidopsis thaliana cFBPase Q9MA79
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应用备注
- Recommended Dilution: 1 : 5 000 with standard ECL (WB).
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说明
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for image of detection please refer to Strand et al. 2000. This antibody does not react with chloroplastic form of FBPase.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 µL of sterile water
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).
: "Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodeling in the Arabidopsis response to phosphate starvation." in: Journal of proteomics, Vol. 94C, pp. 1-22, (2013) (PubMed).
: "Arabidopsis Deficient in Cutin Ferulate encodes a transferase required for feruloylation of ω-hydroxy fatty acids in cutin polyester." in: Plant physiology, Vol. 158, Issue 2, pp. 654-65, (2012) (PubMed).
: "Signal transduction by IRE1-mediated splicing of bZIP50 and other stress sensors in the endoplasmic reticulum stress response of rice." in: The Plant journal : for cell and molecular biology, Vol. 69, Issue 6, pp. 946-56, (2012) (PubMed).
: "Analysis of the Arabidopsis Cytosolic Proteome Highlights Subcellular Partitioning of Central Plant Metabolism." in: Journal of proteome research, (2010) (PubMed).
: "Isobaric tags for relative and absolute quantification- based comparative proteomics reveals the features of plasma membrane-associated proteomes of pollen grains and pollen tubes from Lilium davidii." in: Journal of integrative plant biology, Vol. 52, Issue 12, pp. 1043-58, (2010) (PubMed).
: "Decreased expression of two key enzymes in the sucrose biosynthesis pathway, cytosolic fructose-1,6-bisphosphatase and sucrose phosphate synthase, has remarkably different consequences for photosynthetic carbon metabolism in transgenic Arabidopsis thalian" in: The Plant journal : for cell and molecular biology, Vol. 23, Issue 6, pp. 759-70, (2000) (PubMed).
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: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).
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- cFBPase
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背景
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AGI Code: At1g43670
Fructose-1,6 bisphosphatase (FBPase) (EC=3.1.311) is one of the regulatory enzymes in the sucrose biosynthetic pathway. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism. In photosynthetic tissues cFBPase (cytosolic fructose 1,6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours. Alternative name: D-fructose-1,6-bisphosphate 1-phosphohydrolase -
分子量
- expected: 45 kDa, apparent: 37 kDa (Arabidopsis thaliana)
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UniProt
- Q9MA79
抗原
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