IgG fraction of polyclonal rabbit antiserum to phospholipase A2 from porcine pancreas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
Phospholipase A2 isolated and purified from porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.