Phospholipase A2 from bee venom. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), Block Titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
免疫原
Phospholipase A2 isolated and purified from bee venom. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
溶解方式
Restore by adding 1 mL of sterile distilled water.
浓度
10 mg/mL
缓冲液
PBS, pH 7.2 without preservatives.
储存液
Without preservative
注意事项
Avoid Repeated thawing and freezing.
储存条件
4 °C/-20 °C
储存方法
Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
抗原
PLA2G1B
(Phospholipase A2, Group IB (PLA2G1B))
别名
Phospholipase A2
背景
Phospholipase A2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, a precursor of eicosanoids including prostaglandins and leukotrienes. This enzyme has been proposed to hydrolyze phosphatidylcholine in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci. Phospholipase A2 IIA can exert beneficial action in the context of infectious diseases since recent studies have shown that this enzyme exhibits potent bactericidal effects. Induction of the synthesis of Phospholipase A2 IIA is generally initiated by endotoxin and a limited number of cytokines via paracrine and/or autocrine processes.Synonyms: Allergen Api m I, Phosphatidylcholine 2-acylhydrolase