IgG fraction of polyclonal sheep antiserum to phosphoglyceric mutase from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
纯化方法
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
免疫原
Phosphoglyceric mutase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.